Chemical Cross-linking Mass Spectrometry (XL-MS) is a pivotal technique for investigating protein-protein interactions (PPIs). It employs cross-linkers to chemically conjugate interacting proteins or protein complexes within a defined spatial proximity. Subsequent mass spectrometric analysis of the cross-linked peptides enables the mapping of protein interaction networks and the identification of specific interaction sites. XL-MS can also be integrated with other techniques, such as cryo-electron microscopy (cryo-EM) and X-ray crystallography, for structural biology studies.

Service Advantages

• High Throughput & Rapid Analysis: Enables efficient processing of multiple samples.

• In-cell Cross-linking Capability: Allows for the analysis of interactions within their native cellular environment.

• No Special Chemical Labeling Required: Simplifies sample preparation by eliminating the need for protein tags or labels.

• Captures Transient/Weak Interactions: Cross-linkers covalently link two or more interacting proteins, preserving even short-lived or weak complexes for detection.

Application

• Protein Structure Elucidation

• Study of Interaction Dynamics in Protein-Protein Complexes

• Mapping of Protein-Protein Interaction Interfaces and Sites

Service Workflow

Clients may send pre-crosslinked samples or consult with us to develop a cross-linking strategy before sample submission. We will then perform the complete workflow, including enzymatic digestion, peptide enrichment, mass spectrometry detection, and data analysis, concluding with the delivery of a comprehensive experimental report.